Coding
ZF43.8

Part:BBa_K2549048:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-07)


ZF43.8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 187
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a synthetic engineered zinc finger which can bind to ZF43.8 binding sites with high specificity. Besides, it is flanked by Kozak sequence to facilitate translation.


Source

From IDT (gBlock), human codon optimized